University of Richmond

The linkage between citrate and pH regulation of Malate Dehydrogenase and protein folding

Malate Dehydrogenase (MDH) is a homodimeric oxidoreductase that catalyzes the NAD+/NADH‐dependent reversible oxidation of L‐malate into oxaloacetate.1 The enzyme is involved in the Kreb’s and glyoxylate cycles, as well as the malate‐aspartate shuttle.2 Different isoforms of MDH have been identified including cytosolic, mitochondrial, glyoxysomal, chloroplast, and bacterial, and these isoforms are located in various subcellular areas with specificity for NAD+ or NADP+.3 Each subunit of MDH has two structurally and functionally diverse domains: an NAD+‐binding domain in the amino‐terminal domain

Framing the college debate : insights on Obama's rhetoric through the capabilities approach

President Obama has proposed that the United States increase the number of college graduates by 8 million by 2020 in order to raise national educational standards and to engage in global competition. Obama’s policies and rhetoric frequently describe “education as a pathway to success” and “education as a pathway to jobs,” but fail to emphasize its comprehensive, intrinsic values. America’s national discussion on education needs to shift away from framing higher education merely as pathway to the workforce, and should focus instead on education as a vital part of a well-lived life.

What counts? : legitimizing female role models

The effect of exposure to female role models with gender specific attributes was examined in two studies. In Study 1 both women and men were presented with one of eight stimuli (role model gender: male or female; role model legitimacy attributes: organizational high, organizational low, social high, or social low). Results demonstrated women’s higher preference for female role models and vis‐à‐vis. Regression analyses demonstrated women’s preference for role models in general while men only preferred socially legitimate role models.

Friendship and problem solving : the effect of various situations on co-rumination in emerging adulthood friendships

Co-rumination is the act of negatively discussing problems with another person. The focus of corumination is generally on the negative aspects, or things that cannot be changed as opposed to active problem solving. Co-rumination is positively associated with positive friendship quality as well as internalizing symptoms such as anxiety and depression. Co-rumination is most commonly studied in children and adolescents, but the present study extends this research by looking at the undergraduate population.

The relationship between live coral and macroalgae in South Caicos as influenced by herbivorous fishes

Marine life is the basis for most industries in the Turks and Caicos Islands, located on the outskirts of the Caribbean Sea. Few studies have been carried out to assess the current status of reefs in this region, especially those off the shore of South Caicos Island. The AGRRA v.4.0 methodologies were employed create baseline assessments of stony corals, macroalgae and associated fishes and to explore the relationships between these key reef organisms.

The role of histidines in neurosteroid binding of NMDA GluN2B and D subunits

Implicit in cellular mechanisms essential for learning and memory, NMDA receptors are heterotetrameric members of the family of ionotropic glutamate receptors. Activation of the glutamate-specific receptor subunits results in the opening of ion channels, triggering an excitatory pathway, which can be moderated by certain endogenous and exogenous compounds.

Characterization of microbial community structure in the octocoral Leptogorgia virgulata

The process of spicule formation in L. virgulata results in a drop in pH, which, if left unregulated, could lead to demineralization and subsequent animal death. Carbonic anhydrase has been suggested as a possible pH regulatory mechanism in L. virgulata tissues. This study focuses on an additional hypothetical mechanism of pH regulation involving the production of urease by endosymbiotic bacteria living within L. virgulata tissue. PCR and DGGE are used as culture-independent methods to characterize facets of microbial community structure on L.

Xenopus laevis transgenic lines and their use in the study of lymph heart musculature development

Lymph hearts are pulsatile organs present in lower vertebrates that propel lymph throughout the body and into the venous system, assisting in the maintenance of fluid homeostasis. In organisms such as frogs, several pairs of lymph hearts develop amidst the somites during the early tadpole stages. Due to the unique structure and function of lymph heart musculature—exhibiting characteristics of both skeletal and cardiac muscle—the origin of these cells remains highly controversial.